Difference between revisions of "Part:BBa K3771020"

Revision as of 20:07, 19 October 2021

PpspA-JJU

Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU.

Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria.[1]Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU. JJU converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.

Fig.1 Taurine pathways in E. coli

Usage
We ligased the jju fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.

Characterization

圖片描述

Reference
1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features BBa_K3771020 SequenceAndFeatures

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 <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria.<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">[1]</a>Signal transduction from the outer membrane to the inner membrane activates the <i>pspA</i> promoter, initiating expression of JJU. JJU converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.
 <br>
+  <div style="width=100%; display:flex; align-items: center; justify-content: center;">
+<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:35%;">
+</div>
+<p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p>
     <br><b style="font-size:1.3rem">Usage</b>