Difference between revisions of "Part:BBa K4054100"
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<p>In this year, XJTLU-CHINA characterized pLuxR promoter by this part. As the Figure 1 shows, the composite part consists of the promoter pLuxR (K4054017), the RBS (K4054017)，the coding sequence of eGFP (E0040) and the terminator K4054018.</p> | <p>In this year, XJTLU-CHINA characterized pLuxR promoter by this part. As the Figure 1 shows, the composite part consists of the promoter pLuxR (K4054017), the RBS (K4054017)，the coding sequence of eGFP (E0040) and the terminator K4054018.</p> | ||
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<p>The part was expressed in Cell Free systemm (<i>in vitro</i> system)， which allowed us to meaxure the GFP expression level for different LuxR concentration</p> | <p>The part was expressed in Cell Free systemm (<i>in vitro</i> system)， which allowed us to meaxure the GFP expression level for different LuxR concentration</p> | ||
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Revision as of 19:48, 19 October 2021
pLuxR_T7 RBS_GFP
This is a composite part used for GFP expression. It consist of pLuxR, T7 RBS and ther coding sequence of reporter GFP As it include the component "pLuxR", the activator LuxR is required to start the transcription.
Introduction
In this year, XJTLU-CHINA characterized pLuxR promoter by this part. As the Figure 1 shows, the composite part consists of the promoter pLuxR (K4054017), the RBS (K4054017)，the coding sequence of eGFP (E0040) and the terminator K4054018.
The part was expressed in Cell Free systemm (in vitro system)， which allowed us to meaxure the GFP expression level for different LuxR concentration
Experiment
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 736