Difference between revisions of "Part:BBa K4054100"

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This is a composite part used for GFP expression. It consist of pLuxR, T7 RBS and ther coding sequence of reporter GFP As it include the component "pLuxR", the activator LuxR is required to start the transcription.
 
This is a composite part used for GFP expression. It consist of pLuxR, T7 RBS and ther coding sequence of reporter GFP As it include the component "pLuxR", the activator LuxR is required to start the transcription.
  
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=Usage and Biology=
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<div>
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=Introduction=
  
 
[[File:T--XJTLU-CHINA--LuxR_Activation.JPG|250px|thumb|centro|alt=demostication.|Figure 1. The promoter pLuxR could be activated by AHL and then start the expression of eGFP.]]
 
[[File:T--XJTLU-CHINA--LuxR_Activation.JPG|250px|thumb|centro|alt=demostication.|Figure 1. The promoter pLuxR could be activated by AHL and then start the expression of eGFP.]]
  
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In this year, XJTLU-CHINA characterized pLuxR promoter by this part. As the Figure 1 shows, the composite part consists of the promoter pLuxR (K4054017), the RBS (K4054017)，the coding sequence of eGFP (E0040) and the terminator K4054018.
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<p>In this year, XJTLU-CHINA characterized pLuxR promoter by this part. As the Figure 1 shows, the composite part consists of the promoter pLuxR (K4054017), the RBS (K4054017)，the coding sequence of eGFP (E0040) and the terminator K4054018.</p>
  
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<p>The part was expressed in Cell Free systemm (<i>in vitro</i> system)， which allowed us to meaxure the GFP expression level for different LuxR concentration</p>
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</div>
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<div>
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=Experiment=
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</div>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 19:45, 19 October 2021


pLuxR_T7 RBS_GFP

This is a composite part used for GFP expression. It consist of pLuxR, T7 RBS and ther coding sequence of reporter GFP As it include the component "pLuxR", the activator LuxR is required to start the transcription.

Introduction

demostication.
Figure 1. The promoter pLuxR could be activated by AHL and then start the expression of eGFP.

In this year, XJTLU-CHINA characterized pLuxR promoter by this part. As the Figure 1 shows, the composite part consists of the promoter pLuxR (K4054017), the RBS (K4054017)，the coding sequence of eGFP (E0040) and the terminator K4054018.

The part was expressed in Cell Free systemm (in vitro system)， which allowed us to meaxure the GFP expression level for different LuxR concentration

Experiment

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 736