Difference between revisions of "Part:BBa M10216"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_M10216 short</partinfo>
 
<partinfo>BBa_M10216 short</partinfo>
  
 
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a silver binding peptide {AG4}, circularly permutated ompX {CPompX!} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}
 
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a silver binding peptide {AG4}, circularly permutated ompX {CPompX!} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}
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[[Template:BioE140LSpr09-Streptavidin | Streptavidin binding assay template]]<br>
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[[Template:BioE140LSpr09-Growth | Growth curve assay template]]<br>
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[[Template:BioE140LSpr09-Silver | Silver binding assay template]]<br>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:19, 10 May 2009

{Pbad.rbs.prepro.StrepTag}{AG4}{CPompX!}{dblTerm}

Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a silver binding peptide {AG4}, circularly permutated ompX {CPompX!} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}


Streptavidin binding assay template
Growth curve assay template
Silver binding assay template


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1679
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
    Illegal PstI site found at 1679
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1679
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1679
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005