Difference between revisions of "Part:BBa K3889000"

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<partinfo>BBa_K3889000 short</partinfo>
 
<partinfo>BBa_K3889000 short</partinfo>
  
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Azurite is a Blue Fluorescence Reporter protein from <i>Aequorea victoria</i> and has the following parameters:
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Azurite is a Blue Fluorescence Reporter protein from <i>Aequorea victoria</i> and has the following parameters [1,2]:
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{| class=wikitable
 
{| class=wikitable
 
|-
 
|-
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!Ex λ
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!Ex : Peak excitation wavelength (in nm)
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!Em λ
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!EC (M-1 cm-1)
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!QY
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!Brightness
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!pKa
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!Maturation (min)
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!Lifetime (ns)
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|-
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|383
 
|383
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|-
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!Em: Peak emission wavelength (in nm)
 
|447
 
|447
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|-
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!EC: Extinction Coefficient  (M<sup>-1</sup> cm<sup>-1</sup>)
 
|26,200
 
|26,200
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|-
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!QY: Quantum Yield
 
|0.55
 
|0.55
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|-
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!Brightness
 
|14.41
 
|14.41
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|-
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!pKa
 
|5.0
 
|5.0
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|-
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!Maturation (min)
 
|18.0
 
|18.0
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|-
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!Lifetime (ns)
 
|3.65
 
|3.65
 
|}
 
|}
  
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
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Azurite can be used to report protein production or in systems where fluorescence is required like FRET. In <partinfo>BBa_K3889101</partinfo> we planned to use Azurite as a reporter for P22 c2 repressor production (<partinfo>BBa_K3889101</partinfo>) in <i>Bacillus subtilis </i> 168.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3889000 parameters</partinfo>
 
<partinfo>BBa_K3889000 parameters</partinfo>
 
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==References==
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1. Mena, M. A., Treynor, T. P., Mayo, S. L., & Daugherty, P. S. (2006). Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Nature biotechnology, 24(12), 1569–1571. https://doi.org/10.1038/nbt1264
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2. https://www.fpbase.org/protein/azurite/

Latest revision as of 18:02, 19 October 2021


Azurite_BFP (BFP.B3)

Azurite is a Blue Fluorescence Reporter protein from Aequorea victoria and has the following parameters [1,2]:

Ex : Peak excitation wavelength (in nm) 383
Em: Peak emission wavelength (in nm) 447
EC: Extinction Coefficient (M-1 cm-1) 26,200
QY: Quantum Yield 0.55
Brightness 14.41
pKa 5.0
Maturation (min) 18.0
Lifetime (ns) 3.65



Usage and Biology

Azurite can be used to report protein production or in systems where fluorescence is required like FRET. In BBa_K3889101 we planned to use Azurite as a reporter for P22 c2 repressor production (BBa_K3889101) in Bacillus subtilis 168.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Mena, M. A., Treynor, T. P., Mayo, S. L., & Daugherty, P. S. (2006). Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Nature biotechnology, 24(12), 1569–1571. https://doi.org/10.1038/nbt1264

2. https://www.fpbase.org/protein/azurite/