Difference between revisions of "Part:BBa K3726011"
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For BOH3 | For BOH3 | ||
| − | <img class="img-fluid" src=" | + | <img class="img-fluid" src="https://static.igem.org/mediawiki/parts/thumb/6/6e/T--MADRID_UCM--RBSBOH3.png/800px-T--MADRID_UCM--RBSBOH3.png&sa=D&source=docs&ust=1634668121947000&usg=AOvVaw22VXNiWjll0c5hDBbQGkVd" alt="DESCRIPCIÓN" data-aos="fade-up"> |
As can be observed, the PduP and Slr1192 internal RBS sequences have been designed to pose a moderate strength. Since this part corresponds with a fully functional n-butanol biosynthesis, relative expression of each enzyme required for optimal pathway functioning is controlled by their internal RBS. | As can be observed, the PduP and Slr1192 internal RBS sequences have been designed to pose a moderate strength. Since this part corresponds with a fully functional n-butanol biosynthesis, relative expression of each enzyme required for optimal pathway functioning is controlled by their internal RBS. | ||
Revision as of 17:58, 19 October 2021
CDS_Lv0_BOH_3
This composite part corresponds to a phytobrick compatible MoClo Lv.0 CDS element. This part is an artificial operon designed to express the enzymes CDS_NphT7 (BBa_K3726000), CDS_PhaB^173S (BBa_K3726001), CDS_Ccr (BBa_K3726002), CDS_PhaJ (BBa_K3726003), CDS_PduP (BBa_K3726004), CDS_Slr1192 (BBa_K3726005).
Within this artificial operon each CDS element is preceded by an in-silico designed RBS sequence using DeNovo DNA software. Only the first coding sequence of the operon is not preceded by an RBS sequence which must be assembled during the level 1 MoClo reaction for the expression of the operon in a level 1 transcriptional unit.
This part has been used within the iGEM MADRID_UCM 2021 iGEM team for the expression of a butanol biosynthesis pathway.
The relative transcription initiation strengths of each RBS in the operon context has been calculated with DeNovo DNA Software. Results are depicted in the graphic below.
For BOH3
<img class="img-fluid" src="https://static.igem.org/mediawiki/parts/thumb/6/6e/T--MADRID_UCM--RBSBOH3.png/800px-T--MADRID_UCM--RBSBOH3.png&sa=D&source=docs&ust=1634668121947000&usg=AOvVaw22VXNiWjll0c5hDBbQGkVd" alt="DESCRIPCIÓN" data-aos="fade-up">
As can be observed, the PduP and Slr1192 internal RBS sequences have been designed to pose a moderate strength. Since this part corresponds with a fully functional n-butanol biosynthesis, relative expression of each enzyme required for optimal pathway functioning is controlled by their internal RBS.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614
Illegal PstI site found at 1915
Illegal PstI site found at 4930 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 461
Illegal NheI site found at 3936
Illegal NheI site found at 4164
Illegal PstI site found at 366
Illegal PstI site found at 614
Illegal PstI site found at 1915
Illegal PstI site found at 4930 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 461
Illegal BglII site found at 5275
Illegal BglII site found at 5392
Illegal BamHI site found at 1998
Illegal BamHI site found at 2337
Illegal XhoI site found at 3270 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614
Illegal PstI site found at 1915
Illegal PstI site found at 4930 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614
Illegal PstI site found at 1915
Illegal PstI site found at 4930
Illegal NgoMIV site found at 105
Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 511
Illegal NgoMIV site found at 2593
Illegal AgeI site found at 883
Illegal AgeI site found at 2121 - 1000COMPATIBLE WITH RFC[1000]