Difference between revisions of "Part:BBa K3989004"

 
 
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<partinfo>BBa_K3989004 short</partinfo>
 
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Shong, J., Huang, Y. M., Bystroff, C., & Collins, C. H. (2013). Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS chemical biology, 8(4), 789-795.
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A variant of the Quorum Sensing regulator protein EsaR<a href="https://parts.igem.org/Part:BBa_K2116001"> BBa_K2116001 </a> with the Aspartic acid at position 91 substituted by Glycine.
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===Characterisation===
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===Usage and Biology===
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According to the literature[1], the amino acid substitution has increased the sensitivity of this protein to 3OC6HSL molecule. The result of our characterisation is shown below and more details can be found in part <a href="https://parts.igem.org/Part:BBa_K3989003"> BBa_K3989003 </a>.
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3989004 SequenceAndFeatures</partinfo>
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[[File:21_UZurich_characterisation_plate_reader.jpeg|700px|]]
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<b>Figure 1.</b> Fluorescence intensity measurement by plate reader(96-well plate). The measurements were done every one hour and this is the curve of the last test.
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[[File:21_UZurich_characterisation_facs.png|700px|]]
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<b>Figure 2.</b> Fluorescence intensity measurement by flow cytometry. The samples are taken from the plate, in which the bacteria has been cultured for 7 hours.
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===Sequence and Features===
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<partinfo>BBa_K3989004 SequenceAndFeatures</partinfo>
  
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===References===
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===Functional Parameters===
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<partinfo>BBa_K3989004 parameters</partinfo>
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1) Shong, J., Huang, Y. M., Bystroff, C., & Collins, C. H. (2013). Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS chemical biology, 8(4), 789-795.
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Latest revision as of 16:16, 19 October 2021


EsaR D91G mutant

A variant of the Quorum Sensing regulator protein EsaR BBa_K2116001 with the Aspartic acid at position 91 substituted by Glycine.

Characterisation

According to the literature[1], the amino acid substitution has increased the sensitivity of this protein to 3OC6HSL molecule. The result of our characterisation is shown below and more details can be found in part BBa_K3989003 .

21 UZurich characterisation plate reader.jpeg

Figure 1. Fluorescence intensity measurement by plate reader(96-well plate). The measurements were done every one hour and this is the curve of the last test.


21 UZurich characterisation facs.png

Figure 2. Fluorescence intensity measurement by flow cytometry. The samples are taken from the plate, in which the bacteria has been cultured for 7 hours.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1) Shong, J., Huang, Y. M., Bystroff, C., & Collins, C. H. (2013). Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS chemical biology, 8(4), 789-795.