Difference between revisions of "Part:BBa K3799026:Design"

 
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===Source===
 
===Source===
  
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Csm6 is encoded by several bacteria that use Type III CRISPR-Cas systems. The sequences we use in our characterization are derived from Enterococcus italicus.
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Csm6 is encoded by several bacteria that use Type III CRISPR-Cas systems. The sequences we use in our characterization are derived from Enterococcus italicus.<br>
 +
https://www.uniprot.org/uniprot/E6LHV2
  
 
===References===
 
===References===

Revision as of 15:58, 19 October 2021


Type III-A CRISPR-Cas effector ribonuclease---Csm6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For easy protein purification, his tag with a TEV cut site is added in the 5' end of the coding sequence.


Source

Csm6 is encoded by several bacteria that use Type III CRISPR-Cas systems. The sequences we use in our characterization are derived from Enterococcus italicus.
https://www.uniprot.org/uniprot/E6LHV2

References