Difference between revisions of "Part:BBa M10018"
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_M10018 short</partinfo>
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<partinfo>BBa_M10018 short</partinfo><br>
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This part is a bglbricks basic part for the coding sequence of a synthetic Protein A. Protein A is a protein found in Staphylococcus aureus that is good at binding to IgG antibodies. This part is the engineered Protein A domain found in: PMID: 9294166. This part binds very well to only the constant region (Fc) of IgG.
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'''Background:'''<br>
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Protein A is a protein found in Staphylococcus aureus that is good at binding to IgG antibodies. There's two sites on IgG that it can bind to. The fab and the fc portions. The fab is of the the 2 heads of the y antibody and the fc is the (constant region) tail. Since only the Fc portion is interesting, an effort was made to find the smallest protein domain possible that still binds to the fc portion of IgG. Phage display was used to select for smaller proteins which still bind to IgG which resulted in the development of Z34C which binds to fc, but loses the ability to bind to fab.
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PMID: 9294166
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----
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This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead
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of XbaI and SpeI.  More information about the BglBricks Standard is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard
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Description Page]
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later
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:17, 10 May 2009

{˂Z34C domain˃}
This part is a bglbricks basic part for the coding sequence of a synthetic Protein A. Protein A is a protein found in Staphylococcus aureus that is good at binding to IgG antibodies. This part is the engineered Protein A domain found in: PMID: 9294166. This part binds very well to only the constant region (Fc) of IgG.

Background:
Protein A is a protein found in Staphylococcus aureus that is good at binding to IgG antibodies. There's two sites on IgG that it can bind to. The fab and the fc portions. The fab is of the the 2 heads of the y antibody and the fc is the (constant region) tail. Since only the Fc portion is interesting, an effort was made to find the smallest protein domain possible that still binds to the fc portion of IgG. Phage display was used to select for smaller proteins which still bind to IgG which resulted in the development of Z34C which binds to fc, but loses the ability to bind to fab. PMID: 9294166

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:


[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]