Difference between revisions of "Part:BBa K3805283"
 
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<partinfo>BBa_K3805283 short</partinfo>
 
<partinfo>BBa_K3805283 short</partinfo>
  
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This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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The part is design
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 13:39, 19 October 2021


sgRNA bind to Pbad promoter

This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]