Difference between revisions of "Part:BBa K3805283"
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This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/. | This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/. | ||
Revision as of 13:39, 19 October 2021
sgRNA bind to Pbad promoter
This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]