Difference between revisions of "Part:BBa K3805283"

Revision as of 13:38, 19 October 2021

sgRNA bind to Pbad promoter This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3805283 short</partinfo>
+This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.
-&#34917;&#20805;
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>