Difference between revisions of "Part:BBa M10018:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_M10018 short</partinfo>
 
<partinfo>BBa_M10018 short</partinfo>
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===Source===
 
===Source===
 +
Synthetic
  
−
Wobble Jw0017F / Jw0018R            (133bp, EcoRI/BamHI)  
+
Wobble Jw0017F / Jw0018R            (133bp, EcoRI/BamHI) <br>
−
Sub into pBca9145-Bca1144#5        (EcoRI/BamHI, 2057+910, L)
+
Sub into pBca9145-Bca1144#5        (EcoRI/BamHI, 2057+910, L)<br>
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Product is pBca9145-M10018          {Z34C}
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Product is pBca9145-M10018          {Z34C}<br>
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---------------------------------------------------
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---------------------------------------------------<br>
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Jw0017F Forward construction of Z34C  
+
Jw0017F Forward construction of Z34C <br>
−
CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG
+
CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG<br>
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Jw0018R Reverse construction of Z34C  
+
Jw0018R Reverse construction of Z34C <br>
−
CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG
+
CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG<br>
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+
  
 
===References===
 
===References===
 +
Starovasnik MA, Braisted AC, Wells JA. (1997) Structural mimicry of a native protein by a minimized binding domain. Proceedings National Academy of Science USA. 94:10080-5.

Latest revision as of 02:30, 10 May 2009

{˂Z34C domain˃}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

later


Source

Synthetic

Wobble Jw0017F / Jw0018R (133bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, 2057+910, L)
Product is pBca9145-M10018 {Z34C}


Jw0017F Forward construction of Z34C
CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG
Jw0018R Reverse construction of Z34C
CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG

References

Starovasnik MA, Braisted AC, Wells JA. (1997) Structural mimicry of a native protein by a minimized binding domain. Proceedings National Academy of Science USA. 94:10080-5.