Difference between revisions of "Part:BBa K4083008"
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| − | By inserting this plasmid, we predicted the effective rhamnolipid production by Pseudomonas putida. <!-- Add more about the biology of this part here | + | By inserting this plasmid, we predicted the effective rhamnolipid production by Pseudomonas putida. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:52, 19 October 2021
RPGDuo2 plasmid with nadE and rhlBA genes
This plasmid consists of four parts: RPGDuo2 plasmid; nadE, rhlA, and rhlB coding sequences (https://parts.igem.org/Part:BBa_K4083000, https://parts.igem.org/Part:BBa_K4083004, https://parts.igem.org/Part:BBa_K4083006, https://parts.igem.org/Part:BBa_K4083007).
By inserting this plasmid, we predicted the effective rhamnolipid production by Pseudomonas putida.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 5565
Illegal BamHI site found at 6125
Illegal XhoI site found at 1757
Illegal XhoI site found at 2024
Illegal XhoI site found at 6301 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1949
Illegal NgoMIV site found at 2019
Illegal NgoMIV site found at 2240
Illegal NgoMIV site found at 4400 - 1000COMPATIBLE WITH RFC[1000]