Difference between revisions of "Part:BBa K3796006"
Yang Feiyue (Talk | contribs) |
|||
Line 3: | Line 3: | ||
<partinfo>BBa_K3796006 short</partinfo> | <partinfo>BBa_K3796006 short</partinfo> | ||
− | This part encodes α- Ketoglutarate dehydrogenase(odhA) | + | This part encodes α- Ketoglutarate dehydrogenase(odhA). Our plan is reducing its transcription efficiency to reduce the amount of succinyl coA and increase the amount of glutamate. It is inserted into C.glutamicum ATCC 13032 genome with a changed of RBS. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | The products of coding gene(odhA) can catelyze the conversion of α- Ketoglutarate to succinyl CoA. | |
+ | <br> | ||
+ | By inserting a weak RBS, the expression of odhA can be reduced, the amount of succinyl CoA would be reduced and glutamate would be increased. | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 11:49, 19 October 2021
α- Ketoglutarate dehydrogenase(odhA) from C.glutamicum ATCC 13032
This part encodes α- Ketoglutarate dehydrogenase(odhA). Our plan is reducing its transcription efficiency to reduce the amount of succinyl coA and increase the amount of glutamate. It is inserted into C.glutamicum ATCC 13032 genome with a changed of RBS.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 976
Illegal PstI site found at 203
Illegal PstI site found at 1399
Illegal PstI site found at 1459
Illegal PstI site found at 1498
Illegal PstI site found at 1771
Illegal PstI site found at 3076 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 976
Illegal PstI site found at 203
Illegal PstI site found at 1399
Illegal PstI site found at 1459
Illegal PstI site found at 1498
Illegal PstI site found at 1771
Illegal PstI site found at 3076 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 976
Illegal BglII site found at 467
Illegal BglII site found at 1028 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 976
Illegal PstI site found at 203
Illegal PstI site found at 1399
Illegal PstI site found at 1459
Illegal PstI site found at 1498
Illegal PstI site found at 1771
Illegal PstI site found at 3076 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 976
Illegal PstI site found at 203
Illegal PstI site found at 1399
Illegal PstI site found at 1459
Illegal PstI site found at 1498
Illegal PstI site found at 1771
Illegal PstI site found at 3076
Illegal NgoMIV site found at 237
Illegal NgoMIV site found at 3448
Illegal AgeI site found at 1090 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1800
Illegal BsaI site found at 3324
Illegal BsaI.rc site found at 1228
Illegal BsaI.rc site found at 1294
Illegal BsaI.rc site found at 2302
Illegal BsaI.rc site found at 2345
Illegal BsaI.rc site found at 2494
Illegal SapI.rc site found at 2512