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uTEV3 is a mutant from the original TEV protease. Three amino acids(I138T, S153N, T180A) are mutated in this variant and the catalytic efficiency is higher than the original one and slightly lower than <a href="https://parts.igem.org/Part:BBa_K3989002"> BBa_K3989002</a>. This variant is generated by directed evolution in yeast.
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uTEV3 is a mutant from the original TEV protease. Three amino acids(I138T, S153N, T180A) are mutated in this variant and the catalytic efficiency is higher than the original one and slightly lower than eTEV(<a href="https://parts.igem.org/Part:BBa_K3989002"> BBa_K3989002</a>). This variant is generated by directed evolution in yeast.
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===Properties===
 
===Properties===
 
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The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1.
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The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1. More comparison between this variant and others can be found in the page of <a href="https://parts.igem.org/Part:BBa_K3989002"> BBa_K3989002</a>.
  
 
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3989001 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
 
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<partinfo>BBa_K3989001 parameters</partinfo>
 
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===Reference===
 
===Reference===

Latest revision as of 10:45, 19 October 2021


uTEV3, efficiency-enhanced TEV protease variant

uTEV3 is a mutant from the original TEV protease. Three amino acids(I138T, S153N, T180A) are mutated in this variant and the catalytic efficiency is higher than the original one and slightly lower than eTEV( BBa_K3989002). This variant is generated by directed evolution in yeast.

Properties

The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1. More comparison between this variant and others can be found in the page of BBa_K3989002.

Fig.1 Comparison of the catalytic efficiency between uTEV3 and the original

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

1) Sanchez, M. I., & Ting, A. Y. (2020). Directed evolution improves the catalytic efficiency of TEV protease. Nature methods, 17(2), 167-174.