Difference between revisions of "Part:BBa K3963001"

 
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The plasmid is combined by the Backbone from the pBWB162 plasmid (BBa_K3963000) and cloned by restriction based cloning with the insert beta-Agarase YM01-3 (BBa_K2094002). mCherry protein was cut out. The plasmid still contains the lacI behind lacIq promoter and a kanamycin selection marker.
 
The plasmid is combined by the Backbone from the pBWB162 plasmid (BBa_K3963000) and cloned by restriction based cloning with the insert beta-Agarase YM01-3 (BBa_K2094002). mCherry protein was cut out. The plasmid still contains the lacI behind lacIq promoter and a kanamycin selection marker.
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===Design===
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[[File:T--Heidelberg--beta-agarase--NT--Map.png|thumbnail|center|500px|<b>Figure 1 Plasmid design</b>]]
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===Experiments and results===
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Revision as of 10:11, 19 October 2021


pBAV1k-lacI-Trc-beta-agarase YM01-3

The plasmid is combined by the Backbone from the pBWB162 plasmid (BBa_K3963000) and cloned by restriction based cloning with the insert beta-Agarase YM01-3 (BBa_K2094002). mCherry protein was cut out. The plasmid still contains the lacI behind lacIq promoter and a kanamycin selection marker.

Design

Figure 1 Plasmid design

Experiments and results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2292
    Illegal BamHI site found at 3167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3183
    Illegal AgeI site found at 3220
  • 1000
    COMPATIBLE WITH RFC[1000]