Difference between revisions of "Part:BBa K4032104"

 
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<partinfo>BBa_K4032104 short</partinfo>
 
<partinfo>BBa_K4032104 short</partinfo>
  
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This part contains sequences of fuse protein of amylase and RFP.
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This part is the addition of a lac promoter and double terminator to BBa_K40320xx. Lac promoter and double terminator were used as contained in BBa_J04450.
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This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units.
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Red fluorescence of RFP is observed under the fluorescence microscope. 
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This part was created by In-Fusion method using BBa_J04450 as inverse and BBa_K523006 as insert.
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Fig. 1 method
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Revision as of 09:17, 19 October 2021


lacI+lacZ+RBS+amylase-RFP+double terminator

This part contains sequences of fuse protein of amylase and RFP.

This part is the addition of a lac promoter and double terminator to BBa_K40320xx. Lac promoter and double terminator were used as contained in BBa_J04450.

This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. Red fluorescence of RFP is observed under the fluorescence microscope.

This part was created by In-Fusion method using BBa_J04450 as inverse and BBa_K523006 as insert.

Fig. 1 method


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 977
    Illegal AgeI site found at 2142
    Illegal AgeI site found at 3221
    Illegal AgeI site found at 3333
  • 1000
    COMPATIBLE WITH RFC[1000]