Difference between revisions of "Part:BBa K4003002"
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<partinfo>BBa_K4003002 short</partinfo> | <partinfo>BBa_K4003002 short</partinfo> | ||
− | T7 promoter | + | === profile === |
+ | |||
+ | ==== Name: T7 promoter ==== | ||
+ | ==== Base Pairs: 19bp ==== | ||
+ | ==== Origin: T7 phage, genome ==== | ||
+ | ==== Properties: A promoter for initiation of the transcription. ==== | ||
+ | |||
+ | === Usage and Biology === | ||
+ | The promoter can react specifically to T7 RNA polymerase and is a sequence that starts gene transcription of T7 phage. It is recognized for binding and initiation of the transcription. Placed before a gene, it promotes its transcription. | ||
+ | |||
+ | === Experimental approach === | ||
+ | Production, purification, and SDS-PAGE analysis of recombinant PPM1A | ||
+ | |||
+ | [[File:T--Shanghai Metro Utd--BBa K4005001-figure2b.png|500px|thumb|center|Figure 2b. SDS-PAGE assey of protein and Freeze-dried powder of the ANG protein..]] | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 08:44, 19 October 2021
T7 promoter
profile
Name: T7 promoter
Base Pairs: 19bp
Origin: T7 phage, genome
Properties: A promoter for initiation of the transcription.
Usage and Biology
The promoter can react specifically to T7 RNA polymerase and is a sequence that starts gene transcription of T7 phage. It is recognized for binding and initiation of the transcription. Placed before a gene, it promotes its transcription.
Experimental approach
Production, purification, and SDS-PAGE analysis of recombinant PPM1A
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]