Difference between revisions of "Part:BBa J70026"
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<partinfo>BBa_J70026 short</partinfo> | <partinfo>BBa_J70026 short</partinfo> | ||
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cat gene and promoter from pC194 plasmid, Genbank V01277. | cat gene and promoter from pC194 plasmid, Genbank V01277. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | MIC for E. coli DH10B cells is 4 ug/ml chloramphenicol. This is near background; the cassette appears not to function in E. coli. Horinouchi (1982) says this cassette is inducible. Growth at 0.5 ug/ml for 30 minutes, followed by resistance at 20 ug/ml in E. coli. See part J70304 for a version with the 5' UTR eliminated in an effort to eliminate this behavior. | ||
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Latest revision as of 17:02, 6 May 2009
cat cassette (chloramphenicol resistance)
cat gene and promoter from pC194 plasmid, Genbank V01277.
Usage and Biology
MIC for E. coli DH10B cells is 4 ug/ml chloramphenicol. This is near background; the cassette appears not to function in E. coli. Horinouchi (1982) says this cassette is inducible. Growth at 0.5 ug/ml for 30 minutes, followed by resistance at 20 ug/ml in E. coli. See part J70304 for a version with the 5' UTR eliminated in an effort to eliminate this behavior.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]