Difference between revisions of "Part:BBa K3771078"

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	<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:50%;">		<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:50%;">
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−	<p align="center">圖片描述</p>
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−	<img src="https://2021.igem.org/wiki/images/3/39/T--NCKU_Tainan--CS-PCR.png" style="width:35%;">	+
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−	<p align="center">Fig. 1 Confirmation of <i>cs</i> fragment by PCR. M: Marker; Lane 1: <i>cs</i> (1272 bp)</p>	+
	<br><b style="font-size:1.3rem">Usage</b>		<br><b style="font-size:1.3rem">Usage</b>
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		+	<br>CS was used in in vivo testing of taurine production. The sequence for CS enzyme and <i>trc</i> promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC). <br>
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		+	<div style="width=100%; display:flex; align-items: center; justify-content: center;">
		+	<img src="https://2021.igem.org/wiki/images/a/aa/T--NCKU_Tainan--3.png" style="width:35%;">
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		+	<p>Fig. 2 Taurine production of Ptrc-cdo1 +PlacI-csad +Ptrc-cs in different growth mediums.</p>
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	<br><b style="font-size:1.3rem">Characterization</b>		<br><b style="font-size:1.3rem">Characterization</b>
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		+	<div style="width=100%; display:flex; align-items: center; justify-content: center;">
		+	<img src="https://2021.igem.org/wiki/images/3/39/T--NCKU_Tainan--CS-PCR.png" style="width:35%;">
		+	</div>
		+	<p align="center">Fig. 1 Confirmation of <i>cs</i> fragment by PCR. M: Marker; Lane 1: <i>cs</i> (1272 bp)</p>
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	<div style="width=100%; display:flex; align-items: center; justify-content: center;">		<div style="width=100%; display:flex; align-items: center; justify-content: center;">
	<img src="https://2021.igem.org/wiki/images/e/e3/T--NCKU_Tainan--CS-Vactor-digestion.png" style="width:35%;">		<img src="https://2021.igem.org/wiki/images/e/e3/T--NCKU_Tainan--CS-Vactor-digestion.png" style="width:35%;">

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Revision as of 03:13, 19 October 2021

Ptrc-CS

Description

P_trc-cs is a composite part consisting of the trc promoter and the cs sequences. This part was used in in vivo testing of taurine production. The sequence for cs and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

trc promoter constitutively facilitates the expression of CS enzyme.

Usage

CS was used in in vivo testing of taurine production. The sequence for CS enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Fig. 2 Taurine production of Ptrc-cdo1 +PlacI-csad +Ptrc-cs in different growth mediums.

Characterization

Fig. 1 Confirmation of cs fragment by PCR. M: Marker; Lane 1: cs (1272 bp)

Fig. 2 Confirmation of pSUI fragment by digestion. M: Marker; Lane 1: pSUI (3664 bp)

Fig. 3 Transformation/CS in DH5α

Fig. 4 Confirmation of cs fragment by colony PCR. M: Marker; Lane 1~6: Different colonies of pSUI-P_trc-CS (1272 bp)

Fig. 5 Confirmation of pSUI-P_trc-CS by digestion. M: Marker; Lane 1: Colony of pSUI-P_trc-CS

Fig. 6 Confirmation of the protein expression of CS M: Marker; Lane 1: CS in DH5α with induction (~46 kDa)

References
Sequence and Features