Difference between revisions of "Part:BBa K3493000"

Latest revision as of 01:09, 19 October 2021

G. algens dextranase CDS

CDS of a family 66 dextranase from the psychrophile organism Gelidibacter algens, codon optimized for Escherichia coli.

EDIT 2021-10-18 : We expressed this dextranase at 18 °C for 18 hours with 1 mM IPTG, and we were able to purify it using IMAC chromatography. Using micro Nelson-Somogyi assays, we determined that optimal pH is at 6.5 and the optimal temperature at 40 °C. Since the enzyme is more stable at temperature under 20 °C, we did our assays at 18 °C which was sufficient later on to prove it reduced the viscosity of ropy maple syrup by degrading dextran.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 385
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 385
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 385
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 385
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3493000 short</partinfo>
-CDS of a family 66 dextranase from the psychrophile organism Gelidibacter algens, codon optimized for Escherichia coli..
+CDS of a family 66 dextranase from the psychrophile organism Gelidibacter algens, codon optimized for Escherichia coli.
+EDIT 2021-10-18 : We expressed this dextranase at 18 °C for 18 hours with 1 mM IPTG, and we were able to purify it using IMAC chromatography. Using micro Nelson-Somogyi assays, we determined that optimal pH is at 6.5 and the optimal temperature at 40 °C. Since the enzyme is more stable at temperature under 20 °C, we did our assays at 18 °C which was sufficient later on to prove it reduced the viscosity of ropy maple syrup by degrading dextran.
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