Difference between revisions of "Part:BBa K3771078"

Revision as of 19:54, 18 October 2021

Ptrc-CS

Description

P_trc-cs is a composite part consisting of the trc promoter and the cs sequences. This part was used in in vivo testing of taurine production. The sequence for cs and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

trc promoter constitutively facilitates the expression of CS enzyme.

圖片描述

Fig. 1 Confirmation of cs fragment by PCR. M: Marker; Lane 1: cs (1272 bp)

Usage

Characterization

Fig. 2 Confirmation of pSUI fragment by digestion. M: Marker; Lane 1: pSUI (3664 bp)

Fig. 3 Transformation/CS in DH5α

Fig. 4 Confirmation of cs fragment by colony PCR. M: Marker; Lane 1~6: Different colonies of pSUI-P_trc-CS (1272 bp)

Fig. 5 Confirmation of pSUI-P_trc-CS by digestion. M: Marker; Lane 1: Colony of pSUI-P_trc-CS

Fig. 6 Confirmation of the protein expression of CS M: Marker; Lane 1: CS in DH5α with induction (~46 kDa)

References
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 580

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+<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:50%;">
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 <p align="center">圖片描述</p>