Difference between revisions of "Part:BBa K4020006"
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| − | + | ==Usage and Biology== | |
| + | The DNA sequence encodes for VP16, a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early (IE) genes (HIRAI et al., 2010). The N-terminal region of the protein confers specificity for the IE genes, whilst the C-terminal part contains two functional regions, VP16ad/c and VP16ad/n, that are required for transcriptional activation (Jonker et al., 2005). The residues composing the VP16ad/c subdomain are from 454 to 490. Both subdomains interact with the transcriptional coactivator PC4 and the general transcription factor TFIIBc. Glutamate 476 plays a crucial role in transcriptional activation (Jonker et al., 2005). It was acquired from the DualMembrane Kit 3 (Thaminy et al., 2003). | ||
| + | ==References== | ||
| + | *HIRAI, H., TANI, T., & KIKYO, N. (2010). Structure and functions of powerful transactivators: VP16, MyoD and FoxA. The International Journal of Developmental Biology, 54(11–12), 1589. https://doi.org/10.1387/IJDB.103194HH | ||
| + | *Jonker, H. R. A., Wechselberger, R. W., Boelens, R., Folkers, G. E., & Kaptein, R. (2005). Structural Properties of the Promiscuous VP16 Activation Domain. Biochemistry, 44(3), 827–839. https://doi.org/10.1021/bi0482912 | ||
| + | *Thaminy, S., Auerbach, D., Arnoldo, A., & Stagljar, I. (2003). Identification of Novel ErbB3-Interacting Factors Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System. Genome Research, 13(7), 1744. https://doi.org/10.1101/GR.1276503 | ||
Latest revision as of 19:54, 18 October 2021
Usage and Biology
The DNA sequence encodes for VP16, a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early (IE) genes (HIRAI et al., 2010). The N-terminal region of the protein confers specificity for the IE genes, whilst the C-terminal part contains two functional regions, VP16ad/c and VP16ad/n, that are required for transcriptional activation (Jonker et al., 2005). The residues composing the VP16ad/c subdomain are from 454 to 490. Both subdomains interact with the transcriptional coactivator PC4 and the general transcription factor TFIIBc. Glutamate 476 plays a crucial role in transcriptional activation (Jonker et al., 2005). It was acquired from the DualMembrane Kit 3 (Thaminy et al., 2003).
References
- HIRAI, H., TANI, T., & KIKYO, N. (2010). Structure and functions of powerful transactivators: VP16, MyoD and FoxA. The International Journal of Developmental Biology, 54(11–12), 1589. https://doi.org/10.1387/IJDB.103194HH
- Jonker, H. R. A., Wechselberger, R. W., Boelens, R., Folkers, G. E., & Kaptein, R. (2005). Structural Properties of the Promiscuous VP16 Activation Domain. Biochemistry, 44(3), 827–839. https://doi.org/10.1021/bi0482912
- Thaminy, S., Auerbach, D., Arnoldo, A., & Stagljar, I. (2003). Identification of Novel ErbB3-Interacting Factors Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System. Genome Research, 13(7), 1744. https://doi.org/10.1101/GR.1276503