Difference between revisions of "Part:BBa K3735016"
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<partinfo>BBa_K3735016 short</partinfo> | <partinfo>BBa_K3735016 short</partinfo> | ||
| − | In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of middle intensity | + | In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of middle intensity <i>SefA</i> and low intensity <i>ssuE</i>. After verifying the function of <i>SefA</i> and <i>ssuE</i> separately, we integrate them and obtain nine combinations of different promoter strengths. And the low <i>SefA</i> and the low <i>ssuE</i> Can produce the best SeNPs. |
Revision as of 17:11, 18 October 2021
Coding sequence
In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of middle intensity SefA and low intensity ssuE. After verifying the function of SefA and ssuE separately, we integrate them and obtain nine combinations of different promoter strengths. And the low SefA and the low ssuE Can produce the best SeNPs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 3099
Illegal NheI site found at 3122 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1537
Illegal BglII site found at 2854
Illegal BglII site found at 3288 - 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]