Difference between revisions of "Part:BBa K4092006"

 
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<partinfo>BBa K4092006 short</partinfo>
  
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Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), has a MFE of -26.70000076 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa K4092006 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa K4092006 parameters</partinfo>
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Revision as of 17:08, 18 October 2021

_NOTOC_ No part name specified with partinfo tag.

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), has a MFE of -26.70000076 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.

Sequence and Features No part name specified with partinfo tag.