Difference between revisions of "Part:BBa K3735012"
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<partinfo>BBa_K3735012 short</partinfo>
 
<partinfo>BBa_K3735012 short</partinfo>
  
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In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of high intensity promoter and middle intensity RBS. After verifying the function of sefA and ssuE separately, we integrate them and obtain nine combinations of different promoter strengths. And the low sefA and the low ssuE Can produce the best SeNPs.
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In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of low intensity <i>SefA</i> and high intensity <i>ssuE</i>. After verifying the function of <i>SefA</i> and <i>ssuE</i> separately, we integrate them and obtain nine combinations of different promoter strengths. And the low <i>SefA</i> and the low <i>ssuE</i> Can produce the best SeNPs.
  
  

Revision as of 17:06, 18 October 2021


Coding sequence

In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of low intensity SefA and high intensity ssuE. After verifying the function of SefA and ssuE separately, we integrate them and obtain nine combinations of different promoter strengths. And the low SefA and the low ssuE Can produce the best SeNPs.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1537
    Illegal BglII site found at 2854
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 644
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1151
    Illegal NgoMIV site found at 1574
    Illegal NgoMIV site found at 2675
    Illegal NgoMIV site found at 2924
    Illegal AgeI site found at 671
    Illegal AgeI site found at 1217
    Illegal AgeI site found at 1373
    Illegal AgeI site found at 1514
    Illegal AgeI site found at 1937
    Illegal AgeI site found at 2294
    Illegal AgeI site found at 2465
  • 1000
    COMPATIBLE WITH RFC[1000]