Difference between revisions of "Part:BBa K3735004"
Line 3: Line 3:
 
<partinfo>BBa_K3735004 short</partinfo>
 
<partinfo>BBa_K3735004 short</partinfo>
  
−
In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity (BBa_J23105). With promoter and RBS, the composite part can be directly imported into plasmid and express SefA gene. If you want to learn about the detailed introduction of SefA, you can click the link below.(https://parts.igem.org/Part:BBa_K3735001)
+
In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity (BBa_J23105). With promoter and RBS, the composite part can be directly imported into plasmid and express <i>SefA</i> gene. If you want to learn about the detailed introduction of <i>SefA</i>, you can click the link below.(https://parts.igem.org/Part:BBa_K3735001)
  
 
https://2021.igem.org/wiki/images/2/2a/T--NAU-CHINA--ES.fig.4.png
 
https://2021.igem.org/wiki/images/2/2a/T--NAU-CHINA--ES.fig.4.png

Revision as of 16:54, 18 October 2021


Coding sequence

In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity (BBa_J23105). With promoter and RBS, the composite part can be directly imported into plasmid and express SefA gene. If you want to learn about the detailed introduction of SefA, you can click the link below.(https://parts.igem.org/Part:BBa_K3735001)

T--NAU-CHINA--ES.fig.4.png
Figure.1 growth curve of different promoters in different selenite concentrations


T--NAU-CHINA--ES.fig.5.png
Figure.2 SeNPs output of different promoters in different selenite concentrations


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1537
    Illegal BglII site found at 2854
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 644
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1151
    Illegal NgoMIV site found at 1574
    Illegal NgoMIV site found at 2675
    Illegal NgoMIV site found at 2924
    Illegal AgeI site found at 671
    Illegal AgeI site found at 1217
    Illegal AgeI site found at 1373
    Illegal AgeI site found at 1514
    Illegal AgeI site found at 1937
    Illegal AgeI site found at 2294
    Illegal AgeI site found at 2465
  • 1000
    COMPATIBLE WITH RFC[1000]