Difference between revisions of "Part:BBa K3771077"

Revision as of 16:03, 18 October 2021

Ptrc-CSAD

Description

P_trc-csad is a composite part consisting of the trc promoter and the csad sequences. This part was used in in vivo testing of taurine production. The sequence for csad and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

trc promoter constitutively facilitates the expression of CSAD enzyme.

Usage

Characterization

Fig. 1 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)

Fig. 2 Confirmation of pSUI-Ptrc-CSAD by digestion. M: Marker; Lane 1~3: Different colonies of pSUI-Ptrc-CSAD (3674 bp)

Fig. 3 Transformation/CSAD in DH5α

Fig. 4 Confirmation of protein expression of CSAD. M: Marker; Lane1: whole cell of CSAD in DH5α; Lane2: soluble protein of CSAD in DH5α (~22 kDa)

References
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 337
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 122

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