Difference between revisions of "Part:BBa K4083000"
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[1] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514 | [1] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514 | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:04, 18 October 2021
pRGPDuo2 plasmid for P. putida
Duo2 plasmid is engineered by Rahul Gauttam, Aindrila Mukhopadhyay, and Steven W. Singer (2020)[1]. The plasmid was sent to us by Rahul Gauttam who is currently working as a postdoctoral fellow at Berkeley Lab.
Reference:
[1] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3853
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3880
Illegal BamHI site found at 31
Illegal XhoI site found at 2175 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6480
- 1000COMPATIBLE WITH RFC[1000]