Difference between revisions of "Part:BBa K3927015"

Revision as of 13:26, 18 October 2021

NLS-NucA

Gene encoding for Staphylococcus aureus endonuclease nucA with a nuclear localisation sequence. Improved part based on part BBa_K1159105.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 25
Illegal AgeI site found at 478
1000
COMPATIBLE WITH RFC[1000]

Description

Usage

Design

Characterization

File:T--NUS Singapore--results tab3 5.png

Figure 1: CFU assay results from pGNucA-H(BY4741) and pGNLSNucA-H(BY4741), ratio of colonies counted from plates with the nuclease induced to the colonies counted from the plates with nuclease uninduced. Nuclease with the NLS attached reduces the total number of live colonies on the plate.

Revision as of 00:12, 14 October 2021 (view source) CWChew (Talk \| contribs) ← Older edit		Revision as of 13:26, 18 October 2021 (view source) CWChew (Talk \| contribs) (→‎Characterization) Newer edit →
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	===Characterization===		===Characterization===
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		+	[[File:T--NUS_Singapore--results_tab3_5.png \|800px\|thumb\|center\|Figure 1: CFU assay results from pGNucA-H(BY4741) and pGNLSNucA-H(BY4741), ratio of colonies counted from plates with the nuclease induced to the colonies counted from the plates with nuclease uninduced. Nuclease with the NLS attached reduces the total number of live colonies on the plate.]]