Difference between revisions of "Part:BBa K3805209"

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<partinfo>BBa_K3805209 short</partinfo>
 
<partinfo>BBa_K3805209 short</partinfo>
  
The bistable switch is composed of our target protein,trc promoter(BBa_K3805208), P2 promoter(BBa_I746104), lacI- LVA (BBa_C0012)and mcherry-ompt.When AIP is released, it will act on P2 promoter and start the expression of lacI-LVA and downstream mcherry-ompt. LacI-LVA inhibits trc promoter.When the concentration of AIP decreases, the P2 promoter will be closed, and the inhibition of lacI-LVA on the trc promoter will be relieved.The trc promoter starts the expression of the target protein.
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The bistable switch is composed of trc promoter(BBa_K3805208), P2 promoter(BBa_I746104), lacI- LVA (BBa_C0012)and mcherry-ompt.When AIP is released, it will act on P2 promoter and start the expression of lacI-LVA and downstream mcherry-ompt. LacI-LVA inhibits trc promoter.When the concentration of AIP decreases, the P2 promoter will be closed, and the inhibition of lacI-LVA on the trc promoter will be relieved.The trc promoter starts the expression of the target protein.
  
 
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Revision as of 12:58, 18 October 2021


Bi-stable Switch

The bistable switch is composed of trc promoter(BBa_K3805208), P2 promoter(BBa_I746104), lacI- LVA (BBa_C0012)and mcherry-ompt.When AIP is released, it will act on P2 promoter and start the expression of lacI-LVA and downstream mcherry-ompt. LacI-LVA inhibits trc promoter.When the concentration of AIP decreases, the P2 promoter will be closed, and the inhibition of lacI-LVA on the trc promoter will be relieved.The trc promoter starts the expression of the target protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]