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This part allows This plasmid contains the INTEGRATE system (<a href="https://parts.igem.org/Part:BBa_K3776019"> BBa_K3776019</a>) cloned into the panS-loxP-MCS shuttle vector (<a href="https://parts.igem.org/Part:BBa_K3776009"> BBa_K3776009</a>). <a href="https://parts.igem.org/Part:BBa_K3776006"> BBa_K3776006</a> consists of a feedback insensitive aspartate kinase (<a href="https://parts.igem.org/Part:BBa_K3776005"> BBa_K3776005</a>) for overproduction of lysine, DavB (<a href="https://parts.igem.org/Part:BBa_K3776001"> BBa_K3776001</a>) and DavA (<a href="https://parts.igem.org/Part:BBa_K3776002"> BBa_K3776002</a>) for synthesis of 5-aminovalerate (5AVA) have been cloned as the donor DNA in the INTEGRATE system.
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This part allows genome insertion of genes enabling induced lysine synthesis and 5-aminovalerate (5AVA) synthesis in Synechococcus elongatus UTEX 2973. The INTEGRATE system (<a href="https://parts.igem.org/Part:BBa_K3776019"> BBa_K3776019</a>) that insert three genes as a donor DNA into the genome of S.elongatus UTEX 2973 is cloned into the panS-loxP-MCS shuttle vector (<a href="https://parts.igem.org/Part:BBa_K3776009"> BBa_K3776009</a>). The shuttle vector can be replicated in E.coli for plasmid miniprep and in S.elongatus UTEX 2973 for expression. <a href="https://parts.igem.org/Part:BBa_K3776006"> BBa_K3776006</a>, consisting a feedback insensitive aspartate kinase (<a href="https://parts.igem.org/Part:BBa_K3776005"> BBa_K3776005</a>) for overproduction of lysine, DavB (<a href="https://parts.igem.org/Part:BBa_K3776001"> BBa_K3776001</a>) and DavA (<a href="https://parts.igem.org/Part:BBa_K3776002"> BBa_K3776002</a>) for synthesis of 5AVA, has been cloned as the donor DNA in the INTEGRATE system.
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Latest revision as of 12:47, 18 October 2021
panS-SPIN-davBA-AK
This part allows genome insertion of genes enabling induced lysine synthesis and 5-aminovalerate (5AVA) synthesis in Synechococcus elongatus UTEX 2973. The INTEGRATE system ( BBa_K3776019) that insert three genes as a donor DNA into the genome of S.elongatus UTEX 2973 is cloned into the panS-loxP-MCS shuttle vector ( BBa_K3776009). The shuttle vector can be replicated in E.coli for plasmid miniprep and in S.elongatus UTEX 2973 for expression. BBa_K3776006, consisting a feedback insensitive aspartate kinase ( BBa_K3776005) for overproduction of lysine, DavB ( BBa_K3776001) and DavA ( BBa_K3776002) for synthesis of 5AVA, has been cloned as the donor DNA in the INTEGRATE system.
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Unknown
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 5846 Illegal BsaI.rc site found at 5820 Illegal SapI.rc site found at 8814
