Difference between revisions of "Part:BBa K4088888"
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С-terminal fragment of β-lactamase fused with dCas13.<br/> | С-terminal fragment of β-lactamase fused with dCas13.<br/> | ||
β-Lactamase (AmpR) - <br/> | β-Lactamase (AmpR) - <br/> | ||
| − | C-terminal fragment of β-lactamase - | + | C-terminal fragment of β-lactamase - <br/><br/> |
| − | dCAS13 is a catalytically dead Cas13 enzyme. <br/><br/> | + | dCAS13 is a catalytically dead Cas13 enzyme. The diverse Cas13 family contains at least four known subtypes, including Cas13a (formerly C2c2), which we used. Cas13 uses ~ 64 nucleotide guide RNA to encode target specificity. Cas13 protein complexes with gRNA detect complementary RNA in the sample. Cas13 based detection is specific and can be customized to recognize single nucleotides on the target RNA.<br/><br/> |
Revision as of 12:44, 18 October 2021
dCas13a-Clact
С-terminal fragment of β-lactamase fused with dCas13.
β-Lactamase (AmpR) -
C-terminal fragment of β-lactamase -
dCAS13 is a catalytically dead Cas13 enzyme. The diverse Cas13 family contains at least four known subtypes, including Cas13a (formerly C2c2), which we used. Cas13 uses ~ 64 nucleotide guide RNA to encode target specificity. Cas13 protein complexes with gRNA detect complementary RNA in the sample. Cas13 based detection is specific and can be customized to recognize single nucleotides on the target RNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1580
Illegal BglII site found at 1790
Illegal BglII site found at 2057
Illegal BglII site found at 2822
Illegal XhoI site found at 3751 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]