Difference between revisions of "Part:BBa K3989008"

 
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<partinfo>BBa_K3989008 short</partinfo>
 
<partinfo>BBa_K3989008 short</partinfo>
  
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Shong, J., & Collins, C. H. (2013). Engineering the esaR promoter for tunable quorum sensing-dependent gene expression. ACS synthetic biology, 2(10), 568-575.
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A promoter that can interact with EsaR protein and initiate downstream gene transcription.
  
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===Basic information===
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This part represents a promoter variant with two <i>esa box</i>(position -10 & between -10, -35), which can be bound to EsaR protein. After binding to the box, the EsaR will recruit the RNA polymerase and regulate the downstream transcription. The original promoter: P<sub>esa</sub>R has only one <i>esa box</i> located at position -10 and shows a higher transcription efficiency.
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:40, 18 October 2021


Engineered quorum sensing esaR promoter: PesaR-C

A promoter that can interact with EsaR protein and initiate downstream gene transcription.

Basic information

This part represents a promoter variant with two esa box(position -10 & between -10, -35), which can be bound to EsaR protein. After binding to the box, the EsaR will recruit the RNA polymerase and regulate the downstream transcription. The original promoter: PesaR has only one esa box located at position -10 and shows a higher transcription efficiency. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 281
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]