Difference between revisions of "Part:BBa K3995004"

 
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Pprovoin5_amilGFP
 
Pprovoin5_amilGFP
  
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===Profile===
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====Name: Pprovoin5_amilGFP====
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====Base Pairs: 976 bp====
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====Origin: Synthetic====
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====Properties: A coding sequence for amilGFP.====
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===Usage and Biology===
 
===Usage and Biology===
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Ptat_AtzR is a coding sequence for expressing atzR. Cyanuric acid combination with AtzR can activate amilGFP expression to show the signs of fluorescence. The content of cyanuric acid is detected by detecting the fluorescence concentration. So Ptat_AtzR should be used with Pprovoin5_amilGFP.
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[[File:T--ECNUAS--BBa_K3995005-Figure1.png|500px|thumb|center|Figure1.Genetic design of biosensor.]]
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===Construct Design===
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amilGFP is key functional factors that show the signs of fluorescence which is controlled by a Pprovoin5 promoter (Figure 2). The Pprovoin5_amilGFP is inserted in the pUC57 mini vector to get plasmid A (Figure 3).
  
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====Lane pUC57-amilGFP: Plasmid pUC57-mini-vector-amilGFP digested by Bsa1 and the band at around 750bp (amilGFP: 703bp) was got.====
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====Lane pUC57-Pprovoin5: Plasmid pUC57-kana-mini-Pprovoin5 (2097bp) digested by Bsa1.====
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:26, 18 October 2021


Pprovoin5_amilGFP

Pprovoin5_amilGFP

Profile

Name: Pprovoin5_amilGFP

Base Pairs: 976 bp

Origin: Synthetic

Properties: A coding sequence for amilGFP.

Usage and Biology

Ptat_AtzR is a coding sequence for expressing atzR. Cyanuric acid combination with AtzR can activate amilGFP expression to show the signs of fluorescence. The content of cyanuric acid is detected by detecting the fluorescence concentration. So Ptat_AtzR should be used with Pprovoin5_amilGFP.

Figure1.Genetic design of biosensor.

Construct Design

amilGFP is key functional factors that show the signs of fluorescence which is controlled by a Pprovoin5 promoter (Figure 2). The Pprovoin5_amilGFP is inserted in the pUC57 mini vector to get plasmid A (Figure 3).

Lane pUC57-amilGFP: Plasmid pUC57-mini-vector-amilGFP digested by Bsa1 and the band at around 750bp (amilGFP: 703bp) was got.

Lane pUC57-Pprovoin5: Plasmid pUC57-kana-mini-Pprovoin5 (2097bp) digested by Bsa1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 92
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]