Difference between revisions of "Part:BBa K3562001"
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AHL-acylase with AHLs (Quorum-sensing factor Acyl Homoserine Lactone) degradation activity. | AHL-acylase with AHLs (Quorum-sensing factor Acyl Homoserine Lactone) degradation activity. | ||
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<partinfo>BBa_K3562001 parameters</partinfo> | <partinfo>BBa_K3562001 parameters</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p> | <p> | ||
| − | AHL-acylase is | + | AHL-acylase is an acylase that cleaves AHL by hydrolyzing the amide linkage between the acyl side chain and HSL moiety.(Fig.1) |
</p> | </p> | ||
[[Image:AHL-acylase.png|frame|'''Figure 1''' Mechanism of AHL-acylase]] | [[Image:AHL-acylase.png|frame|'''Figure 1''' Mechanism of AHL-acylase]] | ||
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</p> | </p> | ||
[[Image:AiiO fig2.png|frame|'''Figure 2''' Reduction of AHL concentration during incubation with ''Ochrobactrum'' sp. 44 cell extracts. a: This analysis was performed in duplicates. The mean value and the standard errors (indicated as ±value) were calculated. b: Percentage values indicate the initial concentration of AHL. c: Percentage values indicate the concentration of remaining AHL.[1]]] | [[Image:AiiO fig2.png|frame|'''Figure 2''' Reduction of AHL concentration during incubation with ''Ochrobactrum'' sp. 44 cell extracts. a: This analysis was performed in duplicates. The mean value and the standard errors (indicated as ±value) were calculated. b: Percentage values indicate the initial concentration of AHL. c: Percentage values indicate the concentration of remaining AHL.[1]]] | ||
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===Protocols=== | ===Protocols=== | ||
<p> | <p> | ||
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<p>For the standard curve:</p> | <p>For the standard curve:</p> | ||
<p> (1) Dissolve certain amount of AHL dry powder in chromatographic grade methanol to create a series of AHL solutions with different concentrations</p> | <p> (1) Dissolve certain amount of AHL dry powder in chromatographic grade methanol to create a series of AHL solutions with different concentrations</p> | ||
| − | + | <p>(2) Analyze them by HPLC and draw the standard curve of AHL concentration versus HPLC data.</p> | |
<p>10. Analyze the data from the experiments of purified enzymes with the help of the standard curve</p> | <p>10. Analyze the data from the experiments of purified enzymes with the help of the standard curve</p> | ||
<p>11. Then use excel to draw the Lineweaver-Burk plot and obtain the Km (Michaelis constant) value of the enzymes towards this kind of AHL</p> | <p>11. Then use excel to draw the Lineweaver-Burk plot and obtain the Km (Michaelis constant) value of the enzymes towards this kind of AHL</p> | ||
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===Reference=== | ===Reference=== | ||
[1]Czajkowski R, Krzyzanowska D, Karczewska J, et al., Inactivation of AHLs by Ochrobactrum sp. A44 depends on the activity of a novel class of AHL acylase[J]. Environmental Microbiology Reports, 2011, 3(1):59-68. | [1]Czajkowski R, Krzyzanowska D, Karczewska J, et al., Inactivation of AHLs by Ochrobactrum sp. A44 depends on the activity of a novel class of AHL acylase[J]. Environmental Microbiology Reports, 2011, 3(1):59-68. | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K3562001 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K3562001 parameters</partinfo> | ||
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Latest revision as of 11:52, 18 October 2021
AiiO (with His-tag)
AHL-acylase with AHLs (Quorum-sensing factor Acyl Homoserine Lactone) degradation activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]