Difference between revisions of "Part:BBa K3771004"
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<br>CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC). | <br>CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC). | ||
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| + | <img src="https://2021.igem.org/wiki/images/6/6b/T--NCKU_Tainan--invivo1.png" style="width:35%;"></html> | ||
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<br><b style="font-size:1.3rem">Characterization | <br><b style="font-size:1.3rem">Characterization | ||
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<br>The CDO1 fragment was synthesized by IDT and amplified by PCR. Agarose gel electrophoresis result is shown in Fig. 2.<br> | <br>The CDO1 fragment was synthesized by IDT and amplified by PCR. Agarose gel electrophoresis result is shown in Fig. 2.<br> | ||
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| + | <p>Fig. 2 Confirmation of pSB4KI-Ptrc-CDO1 by digestion.M: Marker; Lane 1~3: Different colonies of pSB4KI-Ptrc-CDO1 (5470 bp)</p> | ||
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| + | <p>Fig. 1 Confirmation of cdo1 fragment by PCR. M: Marker; Lane 1: cdo1 (603 bp)</p> | ||
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<br><b style="font-size:1.3rem">References | <br><b style="font-size:1.3rem">References | ||
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Revision as of 09:56, 18 October 2021
CDO1
Description
CDO1 is part of the L-cysteine sulfinic acid pathway, one of three possible taurine synthesis pathways. CDO1 oxidizes L-cysteine naturally found in the intestine into L-cysteine sulfinic acid, which is later converted to taurine by CSAD.[1]
Usage
CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).
Characterization
The CDO1 fragment was synthesized by IDT and amplified by PCR. Agarose gel electrophoresis result is shown in Fig. 2.
Fig. 2 Confirmation of pSB4KI-Ptrc-CDO1 by digestion.M: Marker; Lane 1~3: Different colonies of pSB4KI-Ptrc-CDO1 (5470 bp)
Fig. 1 Confirmation of cdo1 fragment by PCR. M: Marker; Lane 1: cdo1 (603 bp)
References
Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093
Sequence and Features
Assembly Compatibility:
- 10
COMPATIBLE WITH RFC[10]- 12
COMPATIBLE WITH RFC[12]- 21
COMPATIBLE WITH RFC[21]- 23
COMPATIBLE WITH RFC[23]- 25
INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 447- 1000
COMPATIBLE WITH RFC[1000]