Difference between revisions of "Part:BBa K3875030"

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Reference:<br>
 
Reference:<br>
 
Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912.  
 
Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912.  
 
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https://doi.org/10.1099/00221287-145-10-2903
 
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Revision as of 08:43, 18 October 2021


Usage and Biology

Phyb-mazE-T1
Biology:
Phyb :the promoter of the hyb operon
mazE :antitoxic gene
Design:
We connect MazE with the phyb promoter to ensure that the mazE protein is expressed only under anaerobic conditions, and once the engineered bacteria are exposed to oxygen, drastic changes in oxygen concentration will cause the phyb promoter expression to fail, thus preventing the expression of antitoxins, which in turn will lead to the death of the engineering bacteria
Reference:
Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912. https://doi.org/10.1099/00221287-145-10-2903

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]