Difference between revisions of "Part:BBa K3875010"

 
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<partinfo>BBa_K3875010 short</partinfo>
 
  
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more information will be provided later.
 
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===Usage and Biology===
 
===Usage and Biology===
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<strong>Design：</strong>
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Contained gene:
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<i>pcd</i> (BBa_K3875005),
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<i>p4h</i> (BBa_K3875004),
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<i>trpB</i> <strong>(BBa_K187029)</strong><br>
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This composite part contains three genes, of which <i>p4h</i> and <i>trpB</i> are the genes involved in the last two steps of 5-HTP synthesis. <i>trpB</i> encodes the beta subunit of tryptophan synthase (react as <strong>Figure 1.</strong>).
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<img src="https://static.igem.org/mediawiki/parts/3/3e/T--BUCT--The_reaction_trpB_gene_action.png"width="640px";height="30px"/><br>
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The purpose of inserting genes of PCD and P4H into the plasmid is to construct an artificial MH4 cycle, for which the main form of pterin in <i>E.coli</i> is MH4 [1]. And dihydromonapterin reductase (DHMR) that is from <i>E. coli</i> itself is together with PCD and P4H to form the MH4 cycle (as <strong>Figure. 2</strong>). <br>
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<img src="https://static.igem.org/mediawiki/parts/5/5b/T--BUCT--MH4_Recycle._PCD%2C_pterin-4%CE%B1-carbinolamine_dehydratase%3B_DHMR%2C_dihydromonapterin_reductase%3B_P4H%2C_phenylalanine_4-hydroxylase.png"width="640px";height="30px"/><br>
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Revision as of 07:30, 18 October 2021


Usage and Biology

Design： Contained gene: pcd (BBa_K3875005), p4h (BBa_K3875004), trpB (BBa_K187029)
This composite part contains three genes, of which p4h and trpB are the genes involved in the last two steps of 5-HTP synthesis. trpB encodes the beta subunit of tryptophan synthase (react as Figure 1.).
The purpose of inserting genes of PCD and P4H into the plasmid is to construct an artificial MH4 cycle, for which the main form of pterin in E.coli is MH4 [1]. And dihydromonapterin reductase (DHMR) that is from E. coli itself is together with PCD and P4H to form the MH4 cycle (as Figure. 2).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 409
  • 1000
    COMPATIBLE WITH RFC[1000]