Difference between revisions of "Part:BBa K3875009"
Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
− | |||
− | |||
− | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <html> | ||
+ | |||
+ | <i>trpE</i> | ||
+ | The <i>trpE</i> gene encodes a synthase enzyme called anthranilate synthase | ||
+ | (AS, the catalytic reaction is shown in <strong>Figure 1</strong>), | ||
+ | which is involved in a key step in tryptophan synthesis and is therefore used to enhance the ability of <i>E. coli</i> to synthesize tryptophan.<br> | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/parts/8/8f/T--BUCT--The_reaction_equation_catalyzed_by_AS.png"width="640px";height="30px"/> | ||
+ | |||
+ | This gene is present in part | ||
+ | <strong><a href = "https://parts.igem.org/Part:BBa_K3875006" > BBa_K3875006</a></strong> and | ||
+ | <strong><a href = "https://parts.igem.org/Part:BBa_K3875011" > BBa_K3875011</a></strong>.<br> | ||
+ | |||
+ | It is also known as <i>anth</i>, ECK1258, <i>tryD</i>,<i> tryp-4</i>. | ||
+ | The sequence of <i>trpE </i>can be found in <i>E. coli</i> BW25113. <br> | ||
+ | </html> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 07:24, 18 October 2021
Usage and Biology
trpE
The trpE gene encodes a synthase enzyme called anthranilate synthase
(AS, the catalytic reaction is shown in Figure 1),
which is involved in a key step in tryptophan synthesis and is therefore used to enhance the ability of E. coli to synthesize tryptophan.
This gene is present in part
BBa_K3875006 and
BBa_K3875011.
It is also known as anth, ECK1258, tryD, tryp-4.
The sequence of trpE can be found in E. coli BW25113.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 533
Illegal BglII site found at 594 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]