Difference between revisions of "Part:BBa K3952000"
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<partinfo>BBa_K3952000 short</partinfo>
 
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Located between exon 29 chromosome 11, apolipoprotein B (APOB) is a 1300bp insertion of a transposable LTR element (ERV2-1). This SNP results in a cholesterol deficiency in Bos taurus. The purpose of this part is to serve as a new target for an SNP detection system.
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Located within chromosome 11 of the Bos taurus genome, the APOB gene codes for apolipoprotein B, a protein involved in the transport of cholesterol in the bloodstream. Using the NCBI reference assembly, the APOB gene '''(Gene ID 494004)''' was located within the genomic DNA sequence of the Bos taurus. Studies have reported an inheritable allele within the APOB gene that causes cholesterol deficiency in cattle. This part contains the wild-type APOB gene, meaning the allele is absent.
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===Usage and Biology===
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=='''Allele & Phenotype'''==
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==='''Usage and Biology'''===
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APOB-100 is the  
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The purpose of this part is to serve as a positive control for a Flappase genetic testing system. Therefore the sequence provide is only a fragment of the Bos taurus genome. The sequence was cut in order to only showcase where the allele would be present (exon 5), in addition to the ease of cloning a smaller sequence would provide.
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As previously stated there is no allele present in the sequence.
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3952000 parameters</partinfo>
 
<partinfo>BBa_K3952000 parameters</partinfo>
 
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Revision as of 04:09, 18 October 2021


APOB-100 (Wild Type)

Located within chromosome 11 of the Bos taurus genome, the APOB gene codes for apolipoprotein B, a protein involved in the transport of cholesterol in the bloodstream. Using the NCBI reference assembly, the APOB gene (Gene ID 494004) was located within the genomic DNA sequence of the Bos taurus. Studies have reported an inheritable allele within the APOB gene that causes cholesterol deficiency in cattle. This part contains the wild-type APOB gene, meaning the allele is absent.


Usage and Biology

The purpose of this part is to serve as a positive control for a Flappase genetic testing system. Therefore the sequence provide is only a fragment of the Bos taurus genome. The sequence was cut in order to only showcase where the allele would be present (exon 5), in addition to the ease of cloning a smaller sequence would provide.


As previously stated there is no allele present in the sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]