Difference between revisions of "Part:BBa K3738005:Design"

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===Design Notes===
 
===Design Notes===
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The design of this part was based of part BBa_K2683012, which is an MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide on the N-terminus.  This part has been modified from the original so as to have the polyarginine tag replaced with a histidine tag, to allow for purification as a monomer for capsid assembly in vitro. This part has been codon-optimized for use in E.coli.
+
The design of this part was based off of part BBa_K2683012, which is an MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide on the N-terminus.  This part has been modified from the original so as to have the polyarginine tag replaced with a histidine tag, to allow for purification as a monomer for capsid assembly in vitro. This part has been codon-optimized for use in E.coli.
  
 
This part has been codon optimized for use in E.coli.
 
This part has been codon optimized for use in E.coli.

Revision as of 01:45, 18 October 2021


MS2 Bacteriophage Capsid Protein with N-Terminal Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of this part was based off of part BBa_K2683012, which is an MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide on the N-terminus. This part has been modified from the original so as to have the polyarginine tag replaced with a histidine tag, to allow for purification as a monomer for capsid assembly in vitro. This part has been codon-optimized for use in E.coli.

This part has been codon optimized for use in E.coli.

Source

BBa_K2683012

References