Difference between revisions of "Part:BBa K3838567"
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<partinfo>BBa_K3838567 parameters</partinfo>
 
<partinfo>BBa_K3838567 parameters</partinfo>
 
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===Data:SZU-China 2021 TEAM===
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During the experiment, we used HIs tag to purify and screen our target protein. It was successfully applied in DH5α -PJB, DH5α -PJT and Nissle 1917-PJT.
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[[File:T--SZU-China--BBa K3838222-JBSH3A.png|500px|center]]
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<center>Figure 1. SDS-Page of affinity purification of transformed DH5α-pJB intracellular protein</center>
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[[File:T--SZU-China--BBa K38382613-JTes42A.png|300px|center]]
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<center>Figure 2. SDS-Page of affinity purification of transformed DH5α-pJT intracellular protein</center>
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[[File:T--SZU-China--BBa K38382613-JTes42B.png|300px|center]]
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<center>Figure 3. SDS-Page electrophoretic diagram of Nissle 1917-pJT intracellular protein</center>

Revision as of 01:20, 18 October 2021


6xHis Tag

It is 6xHis tag. A short amino acid peptide consisting of six histidine can be used as a label for affinity chromatography for protein purification. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Data:SZU-China 2021 TEAM

During the experiment, we used HIs tag to purify and screen our target protein. It was successfully applied in DH5α -PJB, DH5α -PJT and Nissle 1917-PJT.

T--SZU-China--BBa K3838222-JBSH3A.png
Figure 1. SDS-Page of affinity purification of transformed DH5α-pJB intracellular protein
T--SZU-China--BBa K38382613-JTes42A.png
Figure 2. SDS-Page of affinity purification of transformed DH5α-pJT intracellular protein
T--SZU-China--BBa K38382613-JTes42B.png
Figure 3. SDS-Page electrophoretic diagram of Nissle 1917-pJT intracellular protein