Difference between revisions of "Part:BBa K3771078"

 
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<partinfo>BBa_K3771078 short</partinfo>
 
<partinfo>BBa_K3771078 short</partinfo>
  
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test
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<br><b style="font-size:1.3rem">Description</b>
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<br><i>P<sub>trc</sub>-cs</i> is a composite part consisting of the <i>trc</i> promoter and the <i>cs</i> sequences. This part was used in <i>in vivo</i> testing of taurine production. The sequence for <i>cs</i> and <i>trc</i> promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC).
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<br><b style="font-size:1.3rem">Biology</b>
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<br>
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<br><i>trc</i> promoter constitutively facilitates the expression of CS enzyme.
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<br>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:35%;">
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</div>
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<p align="center">圖片描述</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/3/39/T--NCKU_Tainan--CS-PCR.png" style="width:35%;">
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</div>
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<p align="center">Fig. 1 Confirmation of <i>cs</i> fragment by PCR. M: Marker; Lane 1: <i>cs</i> (1272 bp)</p>
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<br><b style="font-size:1.3rem">Usage</b>
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<br>
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<br><b style="font-size:1.3rem">Characterization</b>
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<br>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/e/e3/T--NCKU_Tainan--CS-Vactor-digestion.png" style="width:35%;">
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</div>
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<p align="center">Fig. 2 Confirmation of pSUI fragment by digestion. M: Marker; Lane 1: pSUI (3664 bp)</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/3/35/T--NCKU_Tainan--CS-plate%28DH5a%29.png" style="width:35%;">
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</div>
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<p align="center">Fig. 3 Transformation/CS in DH5α</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/9/9b/T--NCKU_Tainan--CS-colony-PCR.png" style="width:35%;">
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</div>
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<p align="center">Fig. 4 Confirmation of cs fragment by colony PCR.
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M: Marker; Lane 1~6: Different colonies of <i>pSUI-P<sub>trc</sub>-CS</i> (1272 bp)
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</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/1/1d/T--NCKU_Tainan--CS-digestion.png" style="width:35%;">
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</div>
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<p align="center">Fig. 5 Confirmation of <i>pSUI-P<sub>trc</sub>-CS</i> by digestion.
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M: Marker; Lane 1: Colony of <i>pSUI-P<sub>trc</sub>-CS</i>
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</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/6/64/T--NCKU_Tainan--CS-PAGE%28DH5a%29.png" style="width:35%;">
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</div>
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<p align="center">Fig. 6 Confirmation of the protein expression of CS
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M: Marker; Lane 1: CS in DH5α with induction (~46 kDa)
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</p>
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<br><b style="font-size:1.3rem">References</b>
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<br>
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</html>
 
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<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 21:24, 17 October 2021


Ptrc-CS


Description

Ptrc-cs is a composite part consisting of the trc promoter and the cs sequences. This part was used in in vivo testing of taurine production. The sequence for cs and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

trc promoter constitutively facilitates the expression of CS enzyme.

圖片描述

Fig. 1 Confirmation of cs fragment by PCR. M: Marker; Lane 1: cs (1272 bp)


Usage

Characterization

Fig. 2 Confirmation of pSUI fragment by digestion. M: Marker; Lane 1: pSUI (3664 bp)

Fig. 3 Transformation/CS in DH5α

Fig. 4 Confirmation of cs fragment by colony PCR. M: Marker; Lane 1~6: Different colonies of pSUI-Ptrc-CS (1272 bp)

Fig. 5 Confirmation of pSUI-Ptrc-CS by digestion. M: Marker; Lane 1: Colony of pSUI-Ptrc-CS

Fig. 6 Confirmation of the protein expression of CS M: Marker; Lane 1: CS in DH5α with induction (~46 kDa)


References
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 580