Difference between revisions of "Part:BBa K3941005:Design"

 
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===Design Notes===
 
===Design Notes===
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This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag was added to 3' end of the sequence to help purification of protein product. A single aminoacid mutation has been studied as Aspartic acid aminoacid in 185 aminoacid position is changed with Serine aminoacid.
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===Source===
 
===Source===
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The source of this part is <i>Trichoderma reesei</i> endoglucanase II gene.
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The EGII gene comes from <i>T. reesei</i>. We codon optimized the sequence and inserted gene in this composite part to express it.
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===References===
 
===References===

Latest revision as of 21:13, 17 October 2021


pET29b+EGII


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 22
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag was added to 3' end of the sequence to help purification of protein product. A single aminoacid mutation has been studied as Aspartic acid aminoacid in 185 aminoacid position is changed with Serine aminoacid.

Source

The source of this part is Trichoderma reesei endoglucanase II gene.

References