Difference between revisions of "Part:BBa K3771076"

 
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<partinfo>BBa_K3771076 short</partinfo>
 
<partinfo>BBa_K3771076 short</partinfo>
  
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<br><b style="font-size:1.3rem">Description</b>
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<br><i>P<sub>lacI</sub>-csad</i> is a composite part consisting of the <i>lacI</i> promoter and the <i>csad</i> sequences. This part was used in <i>in vivo</i> testing of taurine production. The sequence for <i>csad</i> enzyme and <i>lacI</i> promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC).
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<br><b style="font-size:1.3rem">Biology</b>
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<br><i>lacI</i> promoter constitutively facilitates the expression of CSAD enzyme.
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:35%;">
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<p align="center">圖片描述</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/8/86/T--NCKU_Tainan--CSAD-PCR.png" style="width:35%;">
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<p align="center">Fig. 1 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)</p>
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<br><b style="font-size:1.3rem">Usage</b>
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<br><b style="font-size:1.3rem">Characterization</b>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/4/48/T--NCKU_Tainan--CSAD1-Vactor-digestion.png" style="width:35%;">
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<p align="center">Fig. 2 Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)</p>
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<br><b style="font-size:1.3rem">References</b>
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 21:05, 17 October 2021


PlacI-CSAD


Description

PlacI-csad is a composite part consisting of the lacI promoter and the csad sequences. This part was used in in vivo testing of taurine production. The sequence for csad enzyme and lacI promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

lacI promoter constitutively facilitates the expression of CSAD enzyme.

圖片描述

Fig. 1 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)


Usage

Characterization

Fig. 2 Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)


References
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 476
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 261