Difference between revisions of "Part:BBa K3941005"

Revision as of 21:03, 17 October 2021

pET29b+EGII

BBa_K3941005 is a composite part that we used in our expression plasmids (pET29b). We obtain EG2 wild type with this sequence.

This composite part contains 4 parts:

- T7 Promoter (BBa_K3941000)

- LacO (BBa_K1624002)

- EGII (BBa_K3941001)

- T7 Terminator

EGII

This composite part originated from a codon-optimized (for E. coli DH5⍺) version of the endoglucanase (EG) gene that cleaves the internal beta-1,4-glycosidic bonds in cellulose. We optimized the sequence for expression and added a 6XHis at the end. This part contains a mutation to form a different amino acid than wild type. We changed the Cystine from the 99th amino acid to Valine.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 22
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 93
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 22
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 22
1000
COMPATIBLE WITH RFC[1000]

@@ Line 16: / Line 16: @@
+<font size="+1"><b>EGII</b></font>
+This composite part originated from a codon-optimized (for E. coli DH5⍺) version of the endoglucanase (EG) gene that cleaves the internal beta-1,4-glycosidic bonds in cellulose. We optimized the sequence for expression and added a 6XHis at the end.
+This part contains a mutation to form a different amino acid than wild type. We changed the Cystine from the 99th amino acid to Valine.