Difference between revisions of "Part:BBa K3733037"

Latest revision as of 18:00, 17 October 2021

The mutation of thiosulfate sensing system under the control of J23106a

This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS(L547T) is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of P_phsA promoter. In this system, we use J23106a to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.

The function of BBa_K3733037 is confirmed by iGEM 2021 HZAU-China. See this page:https://parts.igem.org/Part:BBa_K3733037:Experience

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1867
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 3065
Illegal AgeI site found at 3188
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1909

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3733037 short</partinfo>
-<p>This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS(L547T) is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of PphsA promoter. In this system, we use J23106a to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.</p>
+<p>This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS(L547T) is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of P<sub>phsA</sub> promoter. In this system, we use J23106a to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.</p>
 <p>
 The function of BBa_K3733037 is confirmed by iGEM 2021 HZAU-China. See this page:https://parts.igem.org/Part:BBa_K3733037:Experience