Difference between revisions of "Part:BBa K3771016"
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<br>Western blot was performed to confirm the expression of <i>OmpA/OprF</i>.<br> | <br>Western blot was performed to confirm the expression of <i>OmpA/OprF</i>.<br> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 17:48, 17 October 2021
PompA-OmpA/OprF
Description
PompA-ompA/oprF is part of the IFN-γ sensing system. ompA promoter regulates the constitutive expression of OmpA/OprF.
Biology
In the IFN-γ sensing system, binding of IFN-γ to the OmpA/OprF chimeric protein activates the pspA promoter, producing the enzyme required for the synthesis of taurine.
Usage
The ompA promoter sequence was amplified from E. coli MG1655, digested, and purified. We ligased ompA promoter and the ompA/oprF gene expression vector pSU and transformed it into DH5α to complete construction of the plasmid. Then, the plasmid was purified and transformed into E. coli ompA mutant strain JW0940.
Characterization
PCR
圖片描述
Western blot was performed to confirm the expression of OmpA/OprF.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1142
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]